Published-Structural determination of model phospholipid membranes by Raman spectroscopy

Sunghee Lee • January 20, 2022

The research group of Dr. Sunghee Lee at Iona College, New Rochelle, NY, has just published a peer-reviewed education article, titled “Structural Determination of Model Phospholipid Membranes by Raman Spectroscopy: Laboratory Experiment”, appearing in Biochemistry and Molecular Biology Education, a publication of Wiley (https://doi.org/10.1002/bmb.21603)

 

This article provides an experimental module designed to understand cell membranes through integrated concepts of chemical structure, molecular interactions, and membrane structural properties, using Raman spectroscopy of model cell membranes.

 

The main analytical tool used to investigate these systems is confocal Raman microspectrometer, an instrument acquired via a National Science Foundation (NSF)-Major Research Instrumentation (MRI) grant award (NSF-MRI-1427705). Since its introduction, the confocal Raman microspectrometer has been a central workforce for the enhancement of research and education at Iona College.

 

Many research papers in peer-reviewed journals have been published in recent years using confocal Raman spectrometer from the research group of Dr. Sunghee Lee, including "Ibuprofen and the Phosphatidylcholine Bilayer: Membrane Water Permeability in the Presence and Absence of Cholesterol" and "Molecular Organization in Mixed SOPC and SDPC Model Membranes: Water Permeability Studies of Polyunsaturated Lipid Bilayer".

 

However, the publication of this article has a special meaning, because, as an educational article, its techniques can be readily implemented to enhance students learning in Chemistry, Biochemistry, Molecular Biology, and related sciences such as Biophysics and Cell Biology, and has great potential for wide dissemination to the education community.

 

This project is a result of diligent work over the course of many years, and is coauthored by five undergraduates, Joseph Giancaspro (’20 Biochemistry), Patrick Scollan ('21 Biochemistry), Juan Rosario ('21 Biochemistry), Elizabeth Miller (’19 Biochemistry), Samuel Braziel (’18 Chemistry), under the guidance of Dr. Sunghee Lee. Samuel Braziel is the founding member of the Raman project, and who spent the most of his undergraduate research time to establish the protocols and initial parameterization, is now full-time employed in industry as an analytical scientist. Both Elizabeth Miller and Joseph Giancaspro, who dedicated themselves to developing optimum conditions of the method, are in Medical School, and Patrick Scollan (MS program in science education) and Juan Rosario (heading to Medical School) improved the application to the cell membrane models. This is truly an amazing harmonious team effort. Congratulations to the team!

Celebrating Our Graduating Seniors: A Joyful End-of-Semester Gathering

By Sunghee Lee • May 6, 2025
As the semester comes to a close, Project Symphony members gathered to celebrate a very special milestone - the graduation of seven dedicated seniors who have been an integral part of our team for many years. Their passion, enthusiasm, and hard work have not only driven their individual growth but have also contributed to the collective success of our group. Together, we’ve achieved so much more than any one of us could alone - a beautiful reminder of the power of collaboration and shared purpose...the true meaning of the "Project Symphony"! We want to extend our heartfelt thanks to these remarkable students for the energy and commitment they brought to the lab every day. Your contributions have truly made a difference, and we are proud to have been part of your journey. As you embark on your next chapter, we wish you all the very best in your future endeavors. Remember, once you are a member of the Project Symphony, you are forever part of our family. We look forward to seeing the incredible things you will accomplish ahead! Congratulations and thank you for everything!

Congratulations to Dr. Giancaspro on Earning His Medical Degree!

By Sunghee Lee • May 5, 2025
We are thrilled to celebrate a remarkable milestone for our former Project Symphony group member, Joe, who officially earned their Medical Doctor (MD) degree today for SUNY Upstate Medical University. This achievement marks the culmination of years of dedication, hard work, and passion for advancing healthcare. During his time with Project Symphony, Joe demonstrated exceptional commitment to research and a genuine curiosity that inspired us all. We have no doubt that these qualities will make him an outstanding physician and a valuable contributor to the medical community. Please join us in congratulating Dr. Giancaspro on this well-deserved accomplishment. We look forward to seeing all the incredible ways they will impact the world of medicine!

Celebrating Excellence: A Big Congrats to the Project Symphony

By Sunghee Lee • April 17, 2025
I’m so excited to congratulate and celebrate the amazing achievements of our undergraduate research group, the Project Symphony, at this year’s Iona Scholars Day! You all worked incredibly hard, and it really showed. The presentations covered a wide range of interesting and impactful projects that not only highlight your academic skills but also your passion for learning and discovery. Each project was a great example of your creativity and dedication. What stood out the most was how engaged and enthusiastic everyone was about their research. Your energy was contagious, and it was clear you’ve really connected with your topics and learned a lot along the way. A huge shoutout to each of you for your fantastic presentations. Your hard work and passion for research really set you apart, and I’m so proud of everything you’ve accomplished. I’m confident you’ll keep doing great things and making a real impact in your fields. Once again, congrats to everyone on a successful Iona Scholars Day!
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