Published-Structural determination of model phospholipid membranes by Raman spectroscopy

Sunghee Lee • January 20, 2022

The research group of Dr. Sunghee Lee at Iona College, New Rochelle, NY, has just published a peer-reviewed education article, titled “Structural Determination of Model Phospholipid Membranes by Raman Spectroscopy: Laboratory Experiment”, appearing in Biochemistry and Molecular Biology Education, a publication of Wiley (https://doi.org/10.1002/bmb.21603)

 

This article provides an experimental module designed to understand cell membranes through integrated concepts of chemical structure, molecular interactions, and membrane structural properties, using Raman spectroscopy of model cell membranes.

 

The main analytical tool used to investigate these systems is confocal Raman microspectrometer, an instrument acquired via a National Science Foundation (NSF)-Major Research Instrumentation (MRI) grant award (NSF-MRI-1427705). Since its introduction, the confocal Raman microspectrometer has been a central workforce for the enhancement of research and education at Iona College.

 

Many research papers in peer-reviewed journals have been published in recent years using confocal Raman spectrometer from the research group of Dr. Sunghee Lee, including "Ibuprofen and the Phosphatidylcholine Bilayer: Membrane Water Permeability in the Presence and Absence of Cholesterol" and "Molecular Organization in Mixed SOPC and SDPC Model Membranes: Water Permeability Studies of Polyunsaturated Lipid Bilayer".

 

However, the publication of this article has a special meaning, because, as an educational article, its techniques can be readily implemented to enhance students learning in Chemistry, Biochemistry, Molecular Biology, and related sciences such as Biophysics and Cell Biology, and has great potential for wide dissemination to the education community.

 

This project is a result of diligent work over the course of many years, and is coauthored by five undergraduates, Joseph Giancaspro (’20 Biochemistry), Patrick Scollan ('21 Biochemistry), Juan Rosario ('21 Biochemistry), Elizabeth Miller (’19 Biochemistry), Samuel Braziel (’18 Chemistry), under the guidance of Dr. Sunghee Lee. Samuel Braziel is the founding member of the Raman project, and who spent the most of his undergraduate research time to establish the protocols and initial parameterization, is now full-time employed in industry as an analytical scientist. Both Elizabeth Miller and Joseph Giancaspro, who dedicated themselves to developing optimum conditions of the method, are in Medical School, and Patrick Scollan (MS program in science education) and Juan Rosario (heading to Medical School) improved the application to the cell membrane models. This is truly an amazing harmonious team effort. Congratulations to the team!

Project Symphony Spotlight: Celebrating Colleen Clarke

By Sunghee Lee • May 18, 2026
We are proud to celebrate the continued success of former Project Symphony member Colleen Clarke, a Ph.D. candiate at Clemson University, who has recently received the first place in the poster session at the Women in the Global Battery Industry's Science Award. Colleen received this prestigious honor for her academic work on the impact of lignin on the transport of chemical properties of sulfonated ionomer composites for all-vanadium redox flow batteries. Colleen exemplifies the curiosity, dedication, and perseverance that Project Symphony strives to nurture in all of its participants. During her time with Project Symphony, Colleen demonstrated a strong commitment to scientific exploration and collaborative learning. Those early experiences helped shape her path toward advanced study and research, where she continues to excel and make meaningful contributions in her field. We are thrilled to see her accomplishments and look forward to following her continued success. Congratulations, Colleen—we are incredibly proud of you!

Celebrating Project Symphony at ISD 2026

By Sunghee Lee • April 24, 2026
On Thursday, April 16, 2026, our Project Symphony members presented their work at the 17th annual Iona Scholars Day, a campus-wide celebration of student scholarship and creative activity. This event showcases faculty-mentored projects across disciplines and highlights the depth of undergraduate research at Iona. This year, Project Symphony members contributed an impressive total of 15 research posters, representing months—and in many cases, years—of sustained effort, curiosity, and collaboration. Their work stood alongside projects from across the university, helping to put chemistry and biophysical research in the spotlight. As the PI of Project Symphony, I am deeply proud of each team member’s dedication, professionalism, and growth. Watching them explain complex ideas with clarity and confidence to faculty, peers, and visitors was truly inspiring. Their posters did more than present data—they told the story of how careful experimentation, persistence, and teamwork lead to new insights. Congratulations again to all of our presenters—you represented Project Symphony, with excellence.

Project Symphony Seniors Lizzie and Jessica Share PFAS Research at ACS National Meeting

By Sunghee Lee • March 27, 2026
We are thrilled to celebrate two of our Project Symphony leaders, Lizzie and Jessica, who presented their research posters at the Spring 2026 ACS National Meeting in Atlanta, GA. They each shared our group’s culminated work on the impact of PFAS (“forever chemicals”) on mammalian and bacterial membranes in a session of the Division of Colloid and Surface Chemistry. Their posters were part of the Biomaterials and Biointerface symposium, which brings together research at the intersection of colloid and surface science and biology. The symposium highlights studies of biological interfaces, exactly where our work on PFAS–membrane interactions fits in, making it a perfect venue for showcasing what Project Symphony has been building toward over the past several years. Lizzie and Jessica presented the group’s work with professionalism, clarity, and confidence, representing Project Symphony to a truly global audience of scientists. As graduating seniors, this was their final undergraduate presentation, and they rose to the occasion. Much of what they shared has already appeared in two peer‑reviewed publications coauthored with other Project Symphony members, underscoring how sustained collaboration in our lab leads to publishable, impactful science. Adding to the celebration, former Project Symphony member Caroline, now a PhD student in chemistry at Georgia Tech University, joined them in Atlanta to cheer them on. Her support reflects one of the most meaningful parts of Project Symphony: a lasting tradition of alumni and current members helping one another grow, both scientifically and personally. For us, their success is more than just a conference highlight—it’s a powerful example of how we, as a group, work together to achieve what once felt unimaginable. From brainstorming experiments and troubleshooting instruments to interpreting complex data, Lizzie and Jessica’s posters reflected the collective effort of many current and past Project Symphony members. Kudos to every Project Symphony member for contributing to this milestone—and special congratulations to Lizzie and Jessica as they take their next professional steps fully prepared for whatever comes next.
Show More